How do you handle unexpected setbacks or failures in protein engineering experiments?

Protein Engineering Scientist Interview Questions

Sample answer to the question

When faced with unexpected setbacks or failures in protein engineering experiments, I take a systematic approach to troubleshoot and identify the root cause of the problem. I start by reviewing the experimental procedure and analyzing the data to understand what went wrong and where. I consult with colleagues and experts in the field to get their insights and perspectives. If necessary, I repeat the experiments with slight modifications to test different hypotheses and assess the impact of potential variables. Throughout this process, I maintain detailed records of my observations and methodologies. By being proactive in addressing setbacks and failures, I can learn from them and adapt my strategies to improve the experimental outcomes.

A more solid answer

When faced with unexpected setbacks or failures in protein engineering experiments, I approach them with a combination of attention to detail, analytical thinking, and collaboration. Firstly, I carefully review the experimental protocol and meticulously analyze the data to identify any potential flaws or inconsistencies. This helps me pinpoint the exact step or variable that may have led to the setback. Secondly, I leverage my analytical and problem-solving skills to brainstorm alternative approaches or modifications to the experimental design. I consult with colleagues and experts in the field to gather different perspectives and insights, fostering a collaborative environment. This collaboration helps me consider different viewpoints and identify innovative solutions to overcome the setback. Lastly, I take ownership of my experiments and seek opportunities to learn from failures. I maintain comprehensive records of my observations and methodologies, allowing me to track progress, make informed adjustments, and continuously improve my approaches.

Why this is a more solid answer:

The solid answer expands on the basic answer by providing specific examples and details to demonstrate the candidate's attention to detail, analytical and problem-solving skills, and ability to work independently and as part of a team. It highlights the candidate's proactive approach in identifying setbacks, collaborating with others, and taking ownership of their experiments. However, the answer can be further improved by incorporating examples of past experiences or projects where the candidate successfully handled unexpected setbacks or failures.

An exceptional answer

When faced with unexpected setbacks or failures in protein engineering experiments, I rely on my strong attention to detail, analytical thinking, and ability to adapt and learn from failures. In one particular project, we encountered a setback when a protein construct failed to express in our standard expression system. To address this, I meticulously reviewed the cloning and expression protocols, identifying a potential issue with codon optimization that affected the protein's expression levels. I collaborated with a computational biologist to design alternative codon-optimized versions of the gene and successfully achieved high expression levels. This experience taught me the importance of thoroughly reviewing experimental protocols and considering other variables that may impact the outcome. I also realized the value of interdisciplinary collaboration in finding innovative solutions to challenges. By adopting a growth mindset, I actively seek opportunities to learn from failures and continually improve my skills and approaches. I maintain detailed records, documenting both successful outcomes and setbacks, which enable me to spot trends and make data-driven decisions in future experiments.

Why this is an exceptional answer:

The exceptional answer goes beyond the solid answer by providing a specific example of how the candidate successfully handled an unexpected setback in a protein engineering project. The candidate demonstrates their attention to detail by meticulously reviewing experimental protocols and their analytical thinking by identifying and addressing the root cause of the setback. They also showcase their ability to collaborate with a computational biologist to find an innovative solution. The answer highlights the candidate's growth mindset and emphasizes the importance of learning from failures. Additionally, the candidate points out the value of maintaining detailed records to inform future decision-making. The answer effectively aligns with the job description's requirements and evaluation areas.

How to prepare for this question

Familiarize yourself with common setbacks and failures in protein engineering experiments, such as low expression levels or protein misfolding.
Review past projects or experiences where you encountered unexpected setbacks or failures and reflect on how you handled them.
Highlight your attention to detail by discussing specific steps you take to analyze experimental data and identify potential flaws.
Emphasize your analytical and problem-solving skills by sharing examples of how you brainstorm alternative approaches or modifications to experimental designs.
Demonstrate your ability to work independently and as part of a team by discussing instances where you sought input from colleagues or experts in the field.
Showcase your adaptability and willingness to learn from failures by discussing how you made adjustments based on past setbacks.

What interviewers are evaluating

Attention to detail
Analytical and problem-solving skills
Ability to work independently and as part of a team