Have you developed any new methodologies or techniques for protein engineering? If so, can you provide an example?

Protein Engineering Scientist Interview Questions

Sample answer to the question

Yes, I have developed new methodologies and techniques for protein engineering. One example is when I was working on a project to optimize a protein for improved stability and binding affinity. I introduced a novel mutagenesis strategy that allowed me to generate a diverse library of protein variants. Then, I used high-throughput screening techniques to identify the variants with the desired properties. This new approach streamlined the protein engineering process and enabled us to identify highly stable and high-affinity variants more efficiently.

A more solid answer

Yes, I have developed innovative methodologies and techniques for protein engineering during my previous role as a Protein Engineer. One significant example is when I incorporated a novel approach called rational design to engineer a protein with improved stability and functionality. I utilized my strong laboratory skills to design and conduct experiments to identify key amino acid residues that contribute to stability. Additionally, I employed computational modeling tools such as Rosetta and FoldX to predict the effects of mutations on protein structure and stability. This integrated approach allowed me to systematically optimize the protein's stability while maintaining its desired functionality. The resulting protein variant exhibited a significant increase in stability without compromising its binding affinity, making it highly suitable for various applications.

Why this is a more solid answer:

The solid answer expands on the basic answer with more specific details and addresses all the evaluation areas and job requirements listed in the job description. It demonstrates the candidate's familiarity with both laboratory techniques and computational modeling tools. However, it could still provide more specific examples of the candidate's work and highlight their problem-solving skills.

An exceptional answer

Absolutely, I consider myself a creative and resourceful Protein Engineer who has developed several impactful methodologies and techniques for protein engineering throughout my career. One notable example is when I pioneered a novel approach known as directed evolution to engineer a protein with enhanced catalytic activity. I designed a library of genetic variants using site-directed mutagenesis and employed a high-throughput screening platform to identify the variants with improved catalytic efficiency. Furthermore, I utilized computational tools like PyRosetta to gain insights into the structural changes caused by the introduced mutations. By iteratively applying this approach and fine-tuning the protein sequence, I successfully achieved a 5-fold increase in catalytic activity compared to the wild-type protein. This breakthrough has opened up new avenues for enzyme engineering and has the potential to revolutionize industrial processes.

Why this is an exceptional answer:

The exceptional answer goes above and beyond by showcasing the candidate's exceptional creativity and impact in protein engineering. It provides a detailed description of a groundbreaking methodology and highlights the candidate's problem-solving skills and familiarity with both laboratory techniques and computational modeling tools. It also emphasizes the candidate's ability to achieve significant improvements in protein functionality. However, it could still benefit from providing more specific examples of the candidate's work in protein engineering.

How to prepare for this question

Stay updated with the latest advancements in protein engineering methodologies and techniques, including both laboratory and computational approaches.
Review your past projects and experiences in protein engineering to identify any unique methodologies or techniques you have developed.
Highlight your problem-solving skills and ability to creatively approach protein engineering challenges during the interview.
Practice explaining your methodologies and techniques clearly and concisely, using specific examples from your previous work.

What interviewers are evaluating

Laboratory skills
Analytical and problem-solving skills
Molecular biology techniques
Experience with computer-aided protein design tools