Tell us about a experience when you encountered a cell culture contamination issue. How did you identify the source of contamination and resolve the problem?

In a previous role, I encountered a cell culture contamination issue while working on a project for developing a new biologic drug. We noticed that some of the cell cultures were exhibiting abnormal growth patterns and contamination. To identify the source of the contamination, we performed a thorough analysis of our aseptic technique, equipment, and reagents. We found that the contamination was originating from a contaminated media bottle that had not been properly sterilized. We immediately removed the contaminated bottle from the lab and implemented stricter sterilization procedures. This resolved the issue, and we were able to continue our cell culture work without further contamination.

During a project in my previous role, I encountered a cell culture contamination issue that required a careful approach to identify and resolve the problem. We noticed irregular growth patterns and contamination in some of the cell cultures, indicating a potential contamination source. To address this, I conducted a thorough investigation, focusing on multiple factors. Firstly, I reviewed our aseptic technique protocols to ensure they were being followed correctly. Secondly, I inspected the equipment and verified that it was properly cleaned and maintained. Lastly, I examined the reagents for any microbial contamination. Through this systematic approach, we identified that a contaminated batch of fetal bovine serum was the source of the contamination. To resolve the issue, we immediately discarded the contaminated serum and acquired a new batch from a trusted supplier. Additionally, we reinforced our aseptic technique training and implemented stricter monitoring of the cell culture environment. These measures successfully resolved the contamination problem, allowing us to continue our cell culture work without further issues.

Encountering a cell culture contamination issue during my previous role was a challenging experience that required a collaborative and multi-faceted approach to resolve. When we noticed irregular growth patterns and contamination in our cell cultures, I immediately formed a team consisting of scientists, technicians, and quality assurance personnel to address the issue. We performed a comprehensive investigation, carefully examining each step of the cell culture process. In collaboration with our quality control department, we conducted microbial analysis of the media, supplements, and equipment to identify the contamination source. Through this rigorous analysis, we discovered that the contamination was originating from a contaminated cell culture incubator. Realizing the severity of the problem, we promptly isolated the affected cultures and thoroughly cleaned and disinfected the incubator. We also implemented stricter aseptic techniques and established a regular maintenance schedule for all equipment. By involving all stakeholders and ensuring open communication throughout the process, we were able to successfully resolve the contamination issue and prevent its recurrence. This experience highlighted the importance of effective collaboration, attention to detail, and continuous improvement in maintaining a high-quality cell culture environment.