JUNIOR LEVEL

Tell us about a time when you had to troubleshoot an issue with laboratory equipment.

Sample answer to the question

In my previous role as a laboratory technician, I encountered an issue with a spectrophotometer during an experiment. The instrument was not giving accurate readings, which was affecting the reliability of our results. I immediately stopped the experiment and began troubleshooting the problem. I checked the calibration of the spectrophotometer, cleaned the cuvettes, and ensured that the wavelength settings were correct. After trying these initial steps, I still could not resolve the issue. So, I consulted the instrument manual and followed the troubleshooting guide provided. It suggested that I check the light source and detector for any obstructions or damage. I carefully inspected both components and found that the light source was faulty. I contacted the manufacturer and arranged for a replacement part. Once the new light source was installed, I performed a series of tests to ensure its functionality and recalibrated the spectrophotometer. This resolved the issue, and we were able to continue the experiment successfully.

A more solid answer

During my time as a Bioassay Analyst, I encountered a situation where the automated pipetting system in the laboratory malfunctioned while performing an assay. The system was not dispensing the correct volumes of reagents, leading to inaccurate results. As a troubleshooting step, I checked the pipetting tips and made sure they were properly attached. I also inspected the tubing and valves for any blockages, but everything appeared to be fine. Realizing that the issue might be related to the software, I contacted the technical support team of the system manufacturer. They guided me through the process of updating the software to the latest version and recalibrating the pipetting system. After following their instructions, the problem was resolved, and the system started dispensing the correct volumes. To ensure the accuracy of the assay, I repeated the experiment with known samples and compared the results to the expected values. This confirmed that the issue was successfully resolved.

Why this is a more solid answer:

The solid answer provides more specific details about the laboratory equipment involved and highlights the candidate's ability to troubleshoot the issue systematically. The candidate demonstrates their problem-solving skills by taking appropriate steps, such as contacting the technical support team, and verifying the resolution of the problem through repeated experiments. However, it could be further improved by linking the troubleshooting process to the candidate's understanding of bioassays and related laboratory techniques.

An exceptional answer

As a Bioassay Analyst, I encountered a challenge while troubleshooting a temperature control issue with a CO2 incubator. During a cell culture experiment, I noticed that the incubator was not maintaining the set temperature, which could affect the growth and viability of the cells. To address this, I first checked the temperature settings and verified that they were correctly programmed. However, the issue persisted. Knowing the importance of maintaining the desired temperature, I decided to investigate further. I examined the thermocouple placement and found that it was not in direct contact with the samples. To improve temperature accuracy, I adjusted the positioning of the thermocouple to ensure proper heat transfer. Additionally, I inspected the humidity control system and discovered a clogged water reservoir, which was affecting the humidity levels inside the incubator. I thoroughly cleaned and replaced the water reservoir, restoring proper humidity control. To validate the effectiveness of the troubleshooting steps, I performed an experiment using temperature-sensitive samples and monitored their growth over time. The results confirmed that the incubator was functioning optimally. This experience reinforced the importance of regular maintenance and proactive troubleshooting to ensure reliable and accurate experimental outcomes.

Why this is an exceptional answer:

The exceptional answer provides a comprehensive description of the troubleshooting process, including specific details about the temperature control issue and the steps taken to identify and resolve the problem. The candidate demonstrates a deep understanding of bioassays and related laboratory techniques by addressing both temperature and humidity control aspects. The candidate's proactive approach and validation of the resolution through an experiment showcase their problem-solving skills and commitment to ensuring reliable experimental outcomes. The answer goes above and beyond the basic and solid answers by providing additional insights into the importance of regular maintenance and proactive troubleshooting.

How to prepare for this question

Familiarize yourself with various laboratory equipment commonly used in bioassay analysis, such as spectrophotometers, automated pipetting systems, and CO2 incubators.
Develop a solid understanding of temperature and humidity control in laboratory equipment, as these aspects can significantly impact experimental results in bioassay analysis.
Review troubleshooting guides and manuals for laboratory equipment to familiarize yourself with the common issues and possible solutions.
Practice describing specific incidents where you encountered issues with laboratory equipment and how you successfully resolved them.
Highlight the importance of proactive problem-solving and the impact it can have on experimental outcomes during your response.

What interviewers are evaluating

Working knowledge of bioassays and related laboratory techniques
Ability to troubleshoot laboratory equipment
Attention to detail and problem-solving skills